SR-B1 involvement in keratinocytes in vitro wound closure

Muresan Ximena Maria, Sticozzi Claudia, Belmonte Giuseppe, Cervellati Franco, Ferrara Francesca, Mary Ann Lila, Valacchi Giuseppe

Research output: Contribution to journalArticle

Abstract

Skin represents the most extended organ of human body, having as main function the protection of our body from outdoor stressors. Its protective ability is compromised when the skin is disrupted as a consequence of mechanical insults. For this purpose, cutaneous tissue is equipped with an efficient and fine mechanism involved in repairing the wounded area. Among the numerous players that take part in the wound healing process, SR-B1 has been recently shown to have a role in keratinocyte re-epithelialization. SR-B1 is a mediator of cholesterol uptake from HDLs, whereas it is implicated in other cellular processes such as vitamins absorption, vesicle trafficking or pathogen identification. The aim of this study was to investigate the mechanisms involved in SR-B1 role in skin wound closure. Our in vitro data demonstrated that SR-B1 influenced keratinocyte proliferation and migration through a downregulation of nuclear cyclin D1 levels and active MMP9 expression respectively possibly in an NF-kB-dependent mechanism. In addition, SR-B1 was also able to modulate keratinocyte morphology into a pro-migratory cytoskeleton rearrangement. The present in vitro study suggests a new role of SRB1 as a possible new key player in cutaneous wound healing mechanism.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume658
DOIs
StatePublished - Nov 15 2018

Keywords

  • Migration
  • Proliferation
  • Skin
  • Wound healing

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Maria, M. X., Claudia, S., Giuseppe, B., Franco, C., Francesca, F., Lila, M. A., & Giuseppe, V. (2018). SR-B1 involvement in keratinocytes in vitro wound closure. Archives of Biochemistry and Biophysics, 658, 1-6. https://doi.org/10.1016/j.abb.2018.09.014