Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

Jeffrey N. Stuart, Nathan G. Hatcher, Xin Zhang, Rhanor Gillette, Jonathan V. Sweedler

Research output: Contribution to journalArticle

Abstract

One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalAnalyst
Volume130
Issue number2
DOIs
StatePublished - Feb 2005

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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