Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

Jeffrey N. Stuart, Nathan G. Hatcher, Xin Zhang, Rhanor Gillette, Jonathan V Sweedler

Research output: Contribution to journalArticle

Abstract

One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalAnalyst
Volume130
Issue number2
DOIs
StatePublished - Feb 1 2005

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Dimers
electrokinesis
Serotonin
Injections
nitric oxide
steel
hydrodynamics
Stainless Steel
Nitric oxide
Hydrodynamics
Neurons
Nitric Oxide
Stainless steel
electrokinetics
measuring
effect
method

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples. / Stuart, Jeffrey N.; Hatcher, Nathan G.; Zhang, Xin; Gillette, Rhanor; Sweedler, Jonathan V.

In: Analyst, Vol. 130, No. 2, 01.02.2005, p. 147-151.

Research output: Contribution to journalArticle

@article{33c9b66d02e84664bc78e447cab0d21d,
title = "Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples",
abstract = "One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.",
author = "Stuart, {Jeffrey N.} and Hatcher, {Nathan G.} and Xin Zhang and Rhanor Gillette and Sweedler, {Jonathan V}",
year = "2005",
month = "2",
day = "1",
doi = "10.1039/b413024c",
language = "English (US)",
volume = "130",
pages = "147--151",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "2",

}

TY - JOUR

T1 - Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

AU - Stuart, Jeffrey N.

AU - Hatcher, Nathan G.

AU - Zhang, Xin

AU - Gillette, Rhanor

AU - Sweedler, Jonathan V

PY - 2005/2/1

Y1 - 2005/2/1

N2 - One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

AB - One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

UR - http://www.scopus.com/inward/record.url?scp=14044251485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14044251485&partnerID=8YFLogxK

U2 - 10.1039/b413024c

DO - 10.1039/b413024c

M3 - Article

C2 - 15665966

AN - SCOPUS:14044251485

VL - 130

SP - 147

EP - 151

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 2

ER -