@inbook{c1cb03269baa45dda6d35edf58512003,
title = "Spheroid Formation of Human Adipose-Derived Stem Cells Using a Liquid Overlay Technique",
abstract = "Compared to two-dimensional monolayer culture, cells cultured in three-dimensional (3D) platforms provide a more biochemically and physiologically relevant environment to study cell{\textendash}cell and cell{\textendash}extracellular matrix interactions in vitro. Using the liquid overlay technique, a scaffold-free method to generate 3D spheroids from human adipose-derived stem cells is described.",
keywords = "3D cell culture, Adipose-derived stem cells (ASCs), Agarose, Liquid overlay technique (LOT), Spheroids",
author = "Sara Al-Ghadban and Bunnell, \{Bruce A.\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3762-3\_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "263--268",
booktitle = "Methods in Molecular Biology",
}