TY - JOUR
T1 - SPEM
T2 - a state-of-the-art instrument for high resolution molecular imaging of small animal organs.
AU - Reis, Marilia Alves dos
AU - Mejia, Jorge
AU - Batista, Ilza Rosa
AU - Barboza, Marycel Rosa Felisa Figols de
AU - Nogueira, Solange Amorim
AU - Wagner, Jairo
AU - Cabral, Francisco Romero
AU - Davoglio, Petrick Marcellus Victorio Mendonça
AU - Abílio, Vanessa Costhek
AU - Fu, Geng
AU - Li, Nan
AU - Meng, Ling Jian
AU - Shih, Ming Chi
AU - Chen, Chin Tu
AU - Amaro Junior, Edson
AU - Bressan, Rodrigo Affonseca
PY - 2012
Y1 - 2012
N2 - To describe the Single Photon Emission Microscope (SPEM), a state-of-the-art instrument for small animal SPECT imaging, and characterize its performance presenting typical images of different animal organs. SPEM consists of two independent imaging devices based on high resolution scintillators, high sensitivity and resolution Electron-Multiplying CCDs and multi-pinhole collimators. During image acquisition, the mouse is placed in a rotational vertical holder between the imaging devices. Subsequently, an appropriate software tool based on the Maximum Likelihood algorithm iteratively produces the volumetric image. Radiopharmaceuticals for imaging kidneys, heart, thyroid and brain were used. The mice were injected with 74 to 148 MBq/0,3mL and scanned for 40 to 80 minutes, 30 to 60 minutes afterwards. During this procedure, the animals remained under ketamine/xilazine anesthesia. SPEM images of different mouse organs are presented, attesting the imaging capabilities of the instrument. SPEM is an innovative technology for small animal SPECT imaging providing high resolution images with appropriate sensitivity for pre-clinical research. Its use with appropriate radiotracers will allow translational investigation of several animal models of human diseases, their pharmacological treatment and the development of potential new therapeutic agents.
AB - To describe the Single Photon Emission Microscope (SPEM), a state-of-the-art instrument for small animal SPECT imaging, and characterize its performance presenting typical images of different animal organs. SPEM consists of two independent imaging devices based on high resolution scintillators, high sensitivity and resolution Electron-Multiplying CCDs and multi-pinhole collimators. During image acquisition, the mouse is placed in a rotational vertical holder between the imaging devices. Subsequently, an appropriate software tool based on the Maximum Likelihood algorithm iteratively produces the volumetric image. Radiopharmaceuticals for imaging kidneys, heart, thyroid and brain were used. The mice were injected with 74 to 148 MBq/0,3mL and scanned for 40 to 80 minutes, 30 to 60 minutes afterwards. During this procedure, the animals remained under ketamine/xilazine anesthesia. SPEM images of different mouse organs are presented, attesting the imaging capabilities of the instrument. SPEM is an innovative technology for small animal SPECT imaging providing high resolution images with appropriate sensitivity for pre-clinical research. Its use with appropriate radiotracers will allow translational investigation of several animal models of human diseases, their pharmacological treatment and the development of potential new therapeutic agents.
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U2 - 10.1590/S1679-45082012000200015
DO - 10.1590/S1679-45082012000200015
M3 - Article
C2 - 23052457
AN - SCOPUS:84888403590
SN - 1679-4508
VL - 10
SP - 209
EP - 215
JO - Einstein (São Paulo, Brazil)
JF - Einstein (São Paulo, Brazil)
IS - 2
ER -