Spectroscopic diffraction phase microscopy

Hoa Pham, Basanta Bhaduri, Huafeng Ding, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review


We present spectroscopic diffraction phase microscopy (sDPM) as a method capable of measuring quantitative phase images at multiple wavelengths. sDPM uses a spatial light modulator at the Fourier plane of a lens to select desired wavelengths from the white light illumination of a grating. The quantitative phase information at different wavelengths allows us to decouple the refractive index and the thickness from the phase shift induced by biological cells. We demonstrate the capability of the setup by dispersion measurements of microsphere beads and RBCs.

Original languageEnglish (US)
Pages (from-to)3438-3440
Number of pages3
JournalOptics Letters
Issue number16
StatePublished - Aug 15 2012
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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