Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization

Aurore Dubuffet; Judith E. Smith; Leellen Solter; M. Alejandra Perotti; Henk R. Braig; Alison M. Dunn

doi:10.1128/AEM.02699-12

Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization

Aurore Dubuffet, Judith E. Smith, Leellen Solter, M. Alejandra Perotti, Henk R. Braig, Alison M. Dunn

Research output: Contribution to journal › Article › peer-review

Abstract

We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni.

Original language	English (US)
Pages (from-to)	385-388
Number of pages	4
Journal	Applied and environmental microbiology
Volume	79
Issue number	1
DOIs	https://doi.org/10.1128/AEM.02699-12
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni.",

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AU - Braig, Henk R.

AU - Dunn, Alison M.

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Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization

Abstract

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