Spatiotemporal fluctuations of protein folding in living cells

Minghao Guo, Martin Gruebele

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Protein folding landscapes and protein-protein interaction landscapes are subject to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena. The resulting spatiotemporal fluctuations in protein folding rates, protein stability, protein structure, protein function, and protein interactions are the subject of a recent generation of experiments, as well as in-cell modeling. We discuss some specific examples of how the cell modulates energy landscapes and leads to fluctuations that may, in some cases, simply be a consequence of statistical physics, but, in other cases, may be exploited by the cell to improve its fitness.

Original languageEnglish (US)
Title of host publicationMolecular Science of Fluctuations Toward Biological Functions
PublisherSpringer Japan
Pages205-219
Number of pages15
ISBN (Electronic)9784431558408
ISBN (Print)9784431558385
DOIs
StatePublished - Jan 1 2016

Fingerprint

Protein folding
protein folding
Protein Folding
Cells
Proteins
cells
proteins
hydrophobic bonding
protein-protein interactions
physics
protein structure
Protein Stability
hydrodynamics
Physics
Hydrodynamics
Static Electricity
Hydrophobic and Hydrophilic Interactions
Electrostatics
energy
Modulation

Keywords

  • Crowding
  • FReI
  • Fluorescence microscopy
  • In-cell folding
  • Quinary interaction
  • Temperature jump

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Guo, M., & Gruebele, M. (2016). Spatiotemporal fluctuations of protein folding in living cells. In Molecular Science of Fluctuations Toward Biological Functions (pp. 205-219). Springer Japan. https://doi.org/10.1007/978-4-431-55840-8_10

Spatiotemporal fluctuations of protein folding in living cells. / Guo, Minghao; Gruebele, Martin.

Molecular Science of Fluctuations Toward Biological Functions. Springer Japan, 2016. p. 205-219.

Research output: Chapter in Book/Report/Conference proceedingChapter

Guo, M & Gruebele, M 2016, Spatiotemporal fluctuations of protein folding in living cells. in Molecular Science of Fluctuations Toward Biological Functions. Springer Japan, pp. 205-219. https://doi.org/10.1007/978-4-431-55840-8_10
Guo M, Gruebele M. Spatiotemporal fluctuations of protein folding in living cells. In Molecular Science of Fluctuations Toward Biological Functions. Springer Japan. 2016. p. 205-219 https://doi.org/10.1007/978-4-431-55840-8_10
Guo, Minghao ; Gruebele, Martin. / Spatiotemporal fluctuations of protein folding in living cells. Molecular Science of Fluctuations Toward Biological Functions. Springer Japan, 2016. pp. 205-219
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