Spatiotemporal fluctuations of protein folding in living cells

Minghao Guo, Martin Gruebele

Research output: Chapter in Book/Report/Conference proceedingChapter


Protein folding landscapes and protein-protein interaction landscapes are subject to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena. The resulting spatiotemporal fluctuations in protein folding rates, protein stability, protein structure, protein function, and protein interactions are the subject of a recent generation of experiments, as well as in-cell modeling. We discuss some specific examples of how the cell modulates energy landscapes and leads to fluctuations that may, in some cases, simply be a consequence of statistical physics, but, in other cases, may be exploited by the cell to improve its fitness.

Original languageEnglish (US)
Title of host publicationMolecular Science of Fluctuations Toward Biological Functions
Number of pages15
ISBN (Electronic)9784431558408
ISBN (Print)9784431558385
StatePublished - Jan 1 2016


  • Crowding
  • FReI
  • Fluorescence microscopy
  • In-cell folding
  • Quinary interaction
  • Temperature jump

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)


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