Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging

Tianyuan Cao, Nydia Morales-Soto, Jin Jia, Nameera F. Baig, Sage J.B. Dunham, Joseph Ellis, Jonathan V Sweedler, Joshua D. Shrout, Paul W. Bohn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microbial community behavior is coupled to a set of genetically-regulated chemical signals that correlate with cell density-the quorum sensing (QS) system-and there is growing appreciation that the QS-regulated behavior of bacteria is chemically, spatially, and temporally complex. In addition, while it has been known for some time that different species use different QS networks, we are beginning to appreciate that different strains of the same bacterial species also differ in their QS networks. Here, we combine mass spectrometric imaging (MSI) and confocal Raman microscopy (CRM) approaches to investigate co-cultures involving different strains (FRD1 and PAO1C) of the same species (Pseudomonas aeruginosa) as well as those involving different species (P. aeruginosa and E. coli). Combining MSI and CRM makes it possible to supersede the limits imposed by individual imaging approaches and enables the spatial mapping of individual bacterial species and their microbial products within a mixed bacterial community growing in situ on surfaces. MSI is used to delineate the secretion of a specific rhamnolipid surfactant as well as alkyl quinolone (AQ) messengers between FRD1 and PAO1C strains of P. aeruginosa, showing that the spatial distribution and production of AQ messengers in PAO1C differs substantially from that of FRD1. In the case of multiple species, CRM is used to show that the prolific secretion of AQs by the PAO1C strain of P. aeruginosa is used to mediate its interaction with cocultured E. coli.

Original languageEnglish (US)
Title of host publicationPhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
EditorsTianhong Dai, Jurgen Popp, Mei X. Wu
PublisherSPIE
ISBN (Electronic)9781510623682
DOIs
StatePublished - Jan 1 2019
EventPhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019 - San Francisco, United States
Duration: Feb 4 2019Feb 5 2019

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10863
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferencePhotonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019
CountryUnited States
CitySan Francisco
Period2/4/192/5/19

Fingerprint

Co-culture
Quorum Sensing
Imaging
Imaging techniques
Microscopic examination
secretions
Confocal
Quinolones
Raman
microscopy
Microscopy
Escherichia coli
Secretion
Escherichia Coli
pseudomonas
Surface-Active Agents
bacteria
Spatial distribution
Bacteria
spatial distribution

Keywords

  • Biofilm
  • Co-culture
  • Confocal Raman microscopy
  • Mass spectrometric imaging
  • Pseudomonas aeruginosa
  • Quinolones

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Cao, T., Morales-Soto, N., Jia, J., Baig, N. F., Dunham, S. J. B., Ellis, J., ... Bohn, P. W. (2019). Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging. In T. Dai, J. Popp, & M. X. Wu (Eds.), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II [108630A] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863). SPIE. https://doi.org/10.1117/12.2501349

Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging. / Cao, Tianyuan; Morales-Soto, Nydia; Jia, Jin; Baig, Nameera F.; Dunham, Sage J.B.; Ellis, Joseph; Sweedler, Jonathan V; Shrout, Joshua D.; Bohn, Paul W.

Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. ed. / Tianhong Dai; Jurgen Popp; Mei X. Wu. SPIE, 2019. 108630A (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10863).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cao, T, Morales-Soto, N, Jia, J, Baig, NF, Dunham, SJB, Ellis, J, Sweedler, JV, Shrout, JD & Bohn, PW 2019, Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging. in T Dai, J Popp & MX Wu (eds), Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II., 108630A, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10863, SPIE, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II 2019, San Francisco, United States, 2/4/19. https://doi.org/10.1117/12.2501349
Cao T, Morales-Soto N, Jia J, Baig NF, Dunham SJB, Ellis J et al. Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging. In Dai T, Popp J, Wu MX, editors, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. SPIE. 2019. 108630A. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2501349
Cao, Tianyuan ; Morales-Soto, Nydia ; Jia, Jin ; Baig, Nameera F. ; Dunham, Sage J.B. ; Ellis, Joseph ; Sweedler, Jonathan V ; Shrout, Joshua D. ; Bohn, Paul W. / Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging. Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II. editor / Tianhong Dai ; Jurgen Popp ; Mei X. Wu. SPIE, 2019. (Proceedings of SPIE - The International Society for Optical Engineering).
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