TY - JOUR
T1 - Spatially dependent alkyl quinolone signaling responses to antibiotics in Pseudomonas aeruginosa swarms
AU - Morales-Soto, Nydia
AU - Dunham, Sage J.B.
AU - Baig, Nameera F.
AU - Ellis, Joseph F.
AU - Madukoma, Chinedu S.
AU - Bohn, Paul W.
AU - Sweedler, Jonathan V.
AU - Shrout, Joshua D.
N1 - Funding Information:
This study was supported in part by NIAID, National Institutes of Health, Grant R01AI113219 (to J.D.S., P.W.B., and J.V.S.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This article was selected as one of our Editors’ Picks. This article contains supporting Experimental procedures and Figs. S1–S8. 1Supported by the Springborn Endowment and the National Science Foun-dation Graduate Research Fellowship Program. 2 Supported by the Springborn Endowment. 3 To whom correspondence should be addressed: Dept. of Civil and Environ-mental Engineering and Earth Sciences, University of Notre Dame, Notre Dame, IN 46556. Tel.: 574-631-1726; E-mail: joshua.shrout@nd.edu.
Publisher Copyright:
© 2018 Michalski and Williams Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2018/6/15
Y1 - 2018/6/15
N2 - There is a general lack of understanding about how communities of bacteria respond to exogenous toxins such as antibiotics. Most of our understanding of community-level stress responses comes from the study of stationary biofilm communities. Although several community behaviors and production of specific biomolecules affecting biofilm development and associated behavior have been described for Pseudomonas aeruginosa and other bacteria, we have little appreciation for the production and dispersal of secreted metabolites within the 2D and 3D spaces they occupy as they colonize, spread, and grow on surfaces. Here we specifically studied the phenotypic responses and spatial variability of alkyl quinolones, including the Pseudomonas quinolone signal (PQS) and members of the alkyl hydroxyquinoline (AQNO) subclass, in P. aeruginosa plate-assay swarming communities. We found that PQS production was not a universal signaling response to antibiotics, as tobramycin elicited an alkyl quinolone response, whereas carbenicillin did not. We also found that PQS and AQNO profiles in response to tobramycin were markedly distinct and influenced these swarms on different spatial scales. At some tobramycin exposures, P. aeruginosa swarms produced alkyl quinolones in the range of 150 M PQS and 400 M AQNO that accumulated as aggregates. Our collective findings show that the distribution of alkyl quinolones can vary by several orders of magnitude within the same swarming community. More notably, our results suggest that multiple intercellular signals acting on different spatial scales can be triggered by one common cue.
AB - There is a general lack of understanding about how communities of bacteria respond to exogenous toxins such as antibiotics. Most of our understanding of community-level stress responses comes from the study of stationary biofilm communities. Although several community behaviors and production of specific biomolecules affecting biofilm development and associated behavior have been described for Pseudomonas aeruginosa and other bacteria, we have little appreciation for the production and dispersal of secreted metabolites within the 2D and 3D spaces they occupy as they colonize, spread, and grow on surfaces. Here we specifically studied the phenotypic responses and spatial variability of alkyl quinolones, including the Pseudomonas quinolone signal (PQS) and members of the alkyl hydroxyquinoline (AQNO) subclass, in P. aeruginosa plate-assay swarming communities. We found that PQS production was not a universal signaling response to antibiotics, as tobramycin elicited an alkyl quinolone response, whereas carbenicillin did not. We also found that PQS and AQNO profiles in response to tobramycin were markedly distinct and influenced these swarms on different spatial scales. At some tobramycin exposures, P. aeruginosa swarms produced alkyl quinolones in the range of 150 M PQS and 400 M AQNO that accumulated as aggregates. Our collective findings show that the distribution of alkyl quinolones can vary by several orders of magnitude within the same swarming community. More notably, our results suggest that multiple intercellular signals acting on different spatial scales can be triggered by one common cue.
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U2 - 10.1074/jbc.RA118.002605
DO - 10.1074/jbc.RA118.002605
M3 - Article
C2 - 29588364
AN - SCOPUS:85048871288
SN - 0021-9258
VL - 293
SP - 9544
EP - 9552
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 24
ER -