Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination

Y. Poitelon, S. Bogni, V. Matafora, G. Della-Flora Nunes, E. Hurley, M. Ghidinelli, B. S. Katzenellenbogen, C. Taveggia, N. Silvestri, A. Bachi, A. Sannino, L. Wrabetz, M. L. Feltri

Research output: Contribution to journalArticlepeer-review


Cell-cell interactions promote juxtacrine signals in specific subcellular domains, which are difficult to capture in the complexity of the nervous system. For example, contact between axons and Schwann cells triggers signals required for radial sorting and myelination. Failure in this interaction causes dysmyelination and axonal degeneration. Despite its importance, few molecules at the axo-glial surface are known. To identify novel molecules in axo-glial interactions, we modified the 'pseudopodia' sub-fractionation system and isolated the projections that glia extend when they receive juxtacrine signals from axons. By proteomics we identified the signalling networks present at the glial-leading edge, and novel proteins, including members of the Prohibitin family. Glial-specific deletion of Prohibitin-2 in mice impairs axo-glial interactions and myelination. We thus validate a novel method to model morphogenesis and juxtacrine signalling, provide insights into the molecular organization of the axo-glial contact, and identify a novel class of molecules in myelination.

Original languageEnglish (US)
Article number8303
JournalNature communications
StatePublished - Sep 18 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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