Spatial light interference microscopy (SLIM)

Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Spatial light interference microscopy (SLIM) is a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry. SLIM combines two classic ideas in light imaging: Zernike's phase contrast microscopy, which renders high contrast intensity images of transparent specimens, and Gabor's holography, where the phase information from the object is recorded. Thus, SLIM reveals the intrinsic contrast of cell structures and, in addition, renders quantitative optical path-length maps across the sample. The resulting topographic accuracy is comparable to that of atomic force microscopy, while the acquisition speed is 1,000 times higher. We illustrate the novel insight into cell dynamics via SLIM by experiments on primary cell cultures from the rat brain. SLIM is implemented as an add-on module to an existing phase contrast microscope, which may prove instrumental in impacting the light microscopy field at a large scale.

Original languageEnglish (US)
Title of host publicationIEEE Photonic Society 24th Annual Meeting, PHO 2011
Number of pages1
StatePublished - Dec 1 2011
Event24th Annual Meeting on IEEE Photonic Society, PHO 2011 - Arlington, VA, United States
Duration: Oct 9 2011Oct 13 2011

Publication series

NameIEEE Photonic Society 24th Annual Meeting, PHO 2011


Other24th Annual Meeting on IEEE Photonic Society, PHO 2011
Country/TerritoryUnited States
CityArlington, VA

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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