Spatial light interference microscopy and fourier transform light scattering for cell and tissue characterization

Zhuo Wang (Inventor), Huafeng Ding (Inventor), Gabriel Popescu (Inventor)

Research output: Patent

Abstract

Methods and apparatus for rendering quantitative phase maps across and through transparent samples. A broadband source is employed in conjunction with an objective, Fourier optics, and a programmable two-dimensional phase modulator to obtain amplitude and phase information in an image plane. Methods, referred to as Fourier transform light scattering (FTLS), measure the angular scattering spectrum of the sample. FTLS combines optical microscopy and light scattering for studying inhomogeneous and dynamic media. FTLS relies on quantifying the optical phase and amplitude associated with a coherent image field and propagating it numerically to the scattering plane. Full angular information, limited only by the microscope objective, is obtained from extremely weak scatterers, such as a single micron-sized particle. A flow cytometer may employ FTLS sorting.
Original languageEnglish (US)
U.S. patent number8184298
Filing date5/21/09
StatePublished - May 22 2012

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