Spatial correlation of confocal raman scattering and secondary ion mass spectrometric molecular images of lignocellulosic materials

Zhen Li, Li Qiang Chu, Jonathan V Sweedler, Paul W. Bohn

Research output: Contribution to journalArticle

Abstract

A detailed chemical and structural understanding of preenzymatic processing of lignocellulosic materials (LCMs) is a key objective in the development of renewable energy. Efficient rendering of biomass components into fermentable substrates for conversion into biofuel feedstocks would benefit greatly from the development of new technologies to provide high-quality, spatially resolved chemical information about LCMs during the various processing states. In an effort to realize this important goal, spatially correlated confocal Raman and mass spectrometric images allow the extraction of three-dimensional information from the perennial grass, Miscanthus x giganteus. An optical microscopy-based landmark registry scheme was developed that allows samples to be transferred between laboratories at different institutions, while retaining the capability to access the same physical regions of the samples. Subsequent to higher resolution imaging via confocal Raman microscopy and secondary ion mass spectrometry (SIMS), laser desorption-ionization mass spectrometry was used to place these regions within the overall sample architecture. Excellent sample registry was evident in the highly correlated Raman and SIMS images. In addition, the correlation of vibrational Raman scattering with mass spectra from specific spatial locations allowed confirmation of the assignment of intracellular globular structures to hemicellulose-rich lignin complexes, an assignment which could only be made tentatively from either image alone.

Original languageEnglish (US)
Pages (from-to)2608-2611
Number of pages4
JournalAnalytical chemistry
Volume82
Issue number7
DOIs
StatePublished - Apr 1 2010

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Molecular mass
Secondary ion mass spectrometry
Raman scattering
Ions
Biofuels
Lignin
Processing
Feedstocks
Ionization
Optical microscopy
Mass spectrometry
Desorption
Microscopic examination
Biomass
Imaging techniques
Lasers
Substrates
hemicellulose

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Spatial correlation of confocal raman scattering and secondary ion mass spectrometric molecular images of lignocellulosic materials. / Li, Zhen; Chu, Li Qiang; Sweedler, Jonathan V; Bohn, Paul W.

In: Analytical chemistry, Vol. 82, No. 7, 01.04.2010, p. 2608-2611.

Research output: Contribution to journalArticle

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