Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean

Hyoun Sub Lim; Tae Seok Ko; Kris N. Lambert; Hong Gi Kim; Schuyler S. Korban; Glen L. Hartman; Leslie L. Domier

doi:10.1016/j.plaphy.2005.08.012

Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean

Hyoun Sub Lim, Tae Seok Ko, Kris N. Lambert, Hong Gi Kim, Schuyler S. Korban, Glen L. Hartman, Leslie L. Domier

Research output: Contribution to journal › Article › peer-review

Abstract

Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and β-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (ρ < 0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). β-glucuronidase (GUS) expression was significantly (ρ < 0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene.

Original language	English (US)
Pages (from-to)	1014-1021
Number of pages	8
Journal	Plant Physiology and Biochemistry
Volume	43
Issue number	10-11
DOIs	https://doi.org/10.1016/j.plaphy.2005.08.012
State	Published - Oct 2005

Keywords

Helper component protease
Post-transcriptional gene silencing
Somatic embryogenesis

ASJC Scopus subject areas

Physiology
Genetics
Plant Science

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10.1016/j.plaphy.2005.08.012

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@article{00868550ce444a499a526f684db3eb72,

title = "Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean",

abstract = "Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and β-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (ρ < 0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). β-glucuronidase (GUS) expression was significantly (ρ < 0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene.",

keywords = "Helper component protease, Post-transcriptional gene silencing, Somatic embryogenesis",

author = "Lim, {Hyoun Sub} and Ko, {Tae Seok} and Lambert, {Kris N.} and Kim, {Hong Gi} and Korban, {Schuyler S.} and Hartman, {Glen L.} and Domier, {Leslie L.}",

note = "Funding Information: This research was supported by the North Central Soybean Research Program and the Agricultural Research Service of the United States Department of Agriculture. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2005",

month = oct,

doi = "10.1016/j.plaphy.2005.08.012",

language = "English (US)",

volume = "43",

pages = "1014--1021",

journal = "Plant Physiology and Biochemistry",

issn = "0981-9428",

publisher = "Elsevier Masson s.r.l.",

number = "10-11",

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T1 - Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean

AU - Lim, Hyoun Sub

AU - Ko, Tae Seok

AU - Lambert, Kris N.

AU - Kim, Hong Gi

AU - Korban, Schuyler S.

AU - Hartman, Glen L.

AU - Domier, Leslie L.

N1 - Funding Information: This research was supported by the North Central Soybean Research Program and the Agricultural Research Service of the United States Department of Agriculture. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2005/10

Y1 - 2005/10

N2 - Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and β-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (ρ < 0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). β-glucuronidase (GUS) expression was significantly (ρ < 0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene.

AB - Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and β-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (ρ < 0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). β-glucuronidase (GUS) expression was significantly (ρ < 0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene.

KW - Helper component protease

KW - Post-transcriptional gene silencing

KW - Somatic embryogenesis

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VL - 43

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EP - 1021

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

IS - 10-11

ER -

Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean

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