Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean

Hyoun Sub Lim, Tae Seok Ko, Kris N. Lambert, Hong Gi Kim, Schuyler S. Korban, Glen L. Hartman, Leslie L. Domier

Research output: Contribution to journalArticlepeer-review

Abstract

Soybean mosaic virus (SMV) helper component protease (HC-Pro), a suppressor of post-transcriptional gene silencing, was evaluated for its ability to enhance production of soybean hygromycin-resistant somatic embryos (HR-SEs), and stabilize transgene expression. Immature soybean cotyledonary explants were co-cultured with Agrobacterium tumefaciens strain KYRT1 harboring either pCAMBIA1302, carrying a hygromycin phosphotransferase gene (hpt) and a gene encoding green fluorescent protein; pCAMBIA1305.1, carrying hpt and β-glucuronidase (uidA) genes; pG2-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G2 HC-Pro; or pG5-HC-Pro, a derivative of pCAMBIA1305.1 containing SMV G5 HC-Pro, but lacking uidA. Significantly (ρ < 0.02) higher numbers of HR-SEs were obtained from explants transformed with Agrobacterium harboring either pG2-HC-Pro or pG5-HC-Pro than with either of the vector controls (pCAMBIA1302 or pCAMBIA1305.1). β-glucuronidase (GUS) expression was significantly (ρ < 0.003) higher in 50-day-old transgenic plants expressing GUS along with SMV-HC-Pro and in SMV-infected GUS transgenic plants than in transgenic plants expressing GUS alone. Together, these data suggest that SMV-HC-Pro enhanced recovery of HR-SEs by suppressing silencing of the hygromycin phosphotransferase gene.

Original languageEnglish (US)
Pages (from-to)1014-1021
Number of pages8
JournalPlant Physiology and Biochemistry
Volume43
Issue number10-11
DOIs
StatePublished - Oct 2005

Keywords

  • Helper component protease
  • Post-transcriptional gene silencing
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean'. Together they form a unique fingerprint.

Cite this