TY - JOUR
T1 - Soy molasses as fermentation substrate for production of butanol using Clostridium beijerinckii BA101
AU - Qureshi, N.
AU - Lolas, A.
AU - Blaschek, H. P.
PY - 2001
Y1 - 2001
N2 - Spray-dried soy molasses (SDSM) contains the sugars dextrose, sucrose, fructose, pinitol, raffinose, verbascose, melibiose, and stachyose. Of the 746 g kg-1 total sugars in SDSM, 434 g kg-1 is fermentable using Clostridium beijerinckii BA101. SDSM was used to produce acetone, butanol, and ethanol (ABE) by C. beijerinckii BA101 in batch cultures. Using 80 g l-1 SDSM, 10.7 g l-1 ABE was produced in P2 medium. Higher concentrations of SDSM resulted in poor solvent production due to the presence of excessive salt and inhibitory components. C. beijerinckii BA101 in SDSM at 80 g l-1 concentration produced 22.8 g l-1 ABE when supplemented with 25.3 g l-1 glucose. SDSM contains 57.4 g kg-1 mineral ash and 2% tri-calcium phosphate. Tri-calcium phosphate up to 43.1 g l-1 was not inhibitory and at a tri-calcium phosphate concentration of 28.8 g l-1, the culture produced more solvents (30.1 g l-1) than the control experiment (23.8 g l-1). In contrast, sodium chloride was a strong inhibitor of C. beijerinckii BA101 cell growth. At a concentration of 10 g l-1 sodium chloride, a maximum cell concentration of 0.6 g l-1 was achieved compared to 1.7 g l-1 in the control experiment. The effects of two salts on specific growth rate constant (μ) and specific rate of ABE production (ν) for C. beijerinckii BA101 were examined.
AB - Spray-dried soy molasses (SDSM) contains the sugars dextrose, sucrose, fructose, pinitol, raffinose, verbascose, melibiose, and stachyose. Of the 746 g kg-1 total sugars in SDSM, 434 g kg-1 is fermentable using Clostridium beijerinckii BA101. SDSM was used to produce acetone, butanol, and ethanol (ABE) by C. beijerinckii BA101 in batch cultures. Using 80 g l-1 SDSM, 10.7 g l-1 ABE was produced in P2 medium. Higher concentrations of SDSM resulted in poor solvent production due to the presence of excessive salt and inhibitory components. C. beijerinckii BA101 in SDSM at 80 g l-1 concentration produced 22.8 g l-1 ABE when supplemented with 25.3 g l-1 glucose. SDSM contains 57.4 g kg-1 mineral ash and 2% tri-calcium phosphate. Tri-calcium phosphate up to 43.1 g l-1 was not inhibitory and at a tri-calcium phosphate concentration of 28.8 g l-1, the culture produced more solvents (30.1 g l-1) than the control experiment (23.8 g l-1). In contrast, sodium chloride was a strong inhibitor of C. beijerinckii BA101 cell growth. At a concentration of 10 g l-1 sodium chloride, a maximum cell concentration of 0.6 g l-1 was achieved compared to 1.7 g l-1 in the control experiment. The effects of two salts on specific growth rate constant (μ) and specific rate of ABE production (ν) for C. beijerinckii BA101 were examined.
KW - ABE
KW - Butanol
KW - C. beijerinckii BA101
KW - SDSM
KW - Sodium chloride
KW - Tri-calcium phosphate
KW - μ
KW - ν
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U2 - 10.1038/sj.jim.7000131
DO - 10.1038/sj.jim.7000131
M3 - Article
C2 - 11494105
AN - SCOPUS:0034907204
SN - 1367-5435
VL - 26
SP - 290
EP - 295
JO - Journal of Industrial Microbiology and Biotechnology
JF - Journal of Industrial Microbiology and Biotechnology
IS - 5
ER -