Soluble expression and one-step purification of recombinant bacillus anthracis protective antigen

David C. Willhite, Steven R. Blanke

Research output: Contribution to journalArticlepeer-review

Abstract

We have greatly simplified production of the Bacillus anthracis protective antigen (PA), the immunoprotective antigen of the anthrax vaccine. By varying induction conditions, we have expressed stable, soluble PA in E. coli, affinity-tagged to facilitate rapid, single-step purification. Recombinant PA exhibited identical properties to B. anthracis PA. Our approach is an attractive strategy for generating purified vaccine antigens that are difficult to express recombinantly.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalProtein and Peptide Letters
Volume5
Issue number5
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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