Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy

Donghua H. Zhou; John J. Shea; Andrew J. Nieuwkoop; W. Trent Franks; Benjamin J. Wylie; Charles Mullen; Dennis Sandoz; Chad M. Rienstra

doi:10.1002/anie.200702905

Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy

Donghua H. Zhou, John J. Shea, Andrew J. Nieuwkoop, W. Trent Franks, Benjamin J. Wylie, Charles Mullen, Dennis Sandoz, Chad M. Rienstra

Research output: Contribution to journal › Article › peer-review

Abstract

Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state ¹H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented).

Original language	English (US)
Pages (from-to)	8380-8383
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	44
DOIs	https://doi.org/10.1002/anie.200702905
State	Published - 2007

Keywords

Indirect proton detection
Magic-angle spinning
NMR spectroscopy
Protein structures

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Online availability

10.1002/anie.200702905

Library availability

Discover UIUC Full Text

Cite this

@article{7ed5d05041094789b17f488dd7ccc716,

title = "Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy",

abstract = "Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented).",

keywords = "Indirect proton detection, Magic-angle spinning, NMR spectroscopy, Protein structures",

author = "Zhou, {Donghua H.} and Shea, {John J.} and Nieuwkoop, {Andrew J.} and Franks, {W. Trent} and Wylie, {Benjamin J.} and Charles Mullen and Dennis Sandoz and Rienstra, {Chad M.}",

year = "2007",

doi = "10.1002/anie.200702905",

language = "English (US)",

volume = "46",

pages = "8380--8383",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley & Sons, Ltd.",

number = "44",

}

TY - JOUR

T1 - Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy

AU - Zhou, Donghua H.

AU - Shea, John J.

AU - Nieuwkoop, Andrew J.

AU - Franks, W. Trent

AU - Wylie, Benjamin J.

AU - Mullen, Charles

AU - Sandoz, Dennis

AU - Rienstra, Chad M.

PY - 2007

Y1 - 2007

N2 - Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented).

AB - Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented).

KW - Indirect proton detection

KW - Magic-angle spinning

KW - NMR spectroscopy

KW - Protein structures

UR - http://www.scopus.com/inward/record.url?scp=36148957871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36148957871&partnerID=8YFLogxK

U2 - 10.1002/anie.200702905

DO - 10.1002/anie.200702905

M3 - Article

C2 - 17907259

AN - SCOPUS:36148957871

SN - 1433-7851

VL - 46

SP - 8380

EP - 8383

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 44

ER -

Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this