Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy

Donghua H. Zhou, John J. Shea, Andrew J. Nieuwkoop, W. Trent Franks, Benjamin J. Wylie, Charles Mullen, Dennis Sandoz, Chad M. Rienstra

Research output: Contribution to journalArticle

Abstract

Spinning a magical web: The combination of fast magic-angle spinning, isotopic dilution, and high magnetic field yields particularly well-resolved solid-state 1H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high-resolution structure of microcrystalline GB1. (Graph Presented).

Original languageEnglish (US)
Pages (from-to)8380-8383
Number of pages4
JournalAngewandte Chemie - International Edition
Volume46
Issue number44
DOIs
StatePublished - 2007

Keywords

  • Indirect proton detection
  • Magic-angle spinning
  • NMR spectroscopy
  • Protein structures

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy'. Together they form a unique fingerprint.

  • Cite this

    Zhou, D. H., Shea, J. J., Nieuwkoop, A. J., Franks, W. T., Wylie, B. J., Mullen, C., Sandoz, D., & Rienstra, C. M. (2007). Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy. Angewandte Chemie - International Edition, 46(44), 8380-8383. https://doi.org/10.1002/anie.200702905