Soft lithography-based nano-well SERS substrate for label-free biomolecular detection chip

Gang Logan Liu, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed soft lithography-based nano-well surface-enhancement Raman scattering (SERS) substrates with sub-1 pM detection sensitivity, which can be useful for label-free genomics, proteomics, or environmental monitoring biosensors. We batch-fabricated pillarlike nanostructures on a silicon wafer (i.e. master) [1] to replicate the complementary nanowell structures using polydimethylsiloxane (PDMS) by soft lithography, and deposited Ag thin film on the nanostructured PDMS. A glass substrate is used as an optical window and to form microfluidic channels integrated with the Ag/PDMS nano-well substrate. SERS spectra of the Rhodamine 6G (R6G) and adenosine molecules in microfluidic channels are detected on the nano-well SERS substrates with only 300 μW excitation power. The Raman scattering signal enhancement on the Ag/PDMS nano-well SERS substrate is more than 107 times higher than that on the smooth silver layer on PDMS. The present successful label-free detection of sub-picomolar level adenosine molecules is important to future studies of low concentration nucleic acid reactions such as hybridization, denaturization, nuclease, and transcription in microfluidics. The monolithic integration of the Ag/PDMS nano-well in microfluidics promises the label-free detection of singlemolecular level biochemical reactions in multiplexed aqueous environments.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1003-1005
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • DNA bases
  • Nanomolding
  • PDMS
  • SERS

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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