We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid sequences in a liquid test sample and compared performance against a conventional laboratory fluorimeter. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route toward portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.

Original languageEnglish (US)
Pages (from-to)8805-8813
Number of pages9
JournalAnalytical Chemistry
Issue number17
StatePublished - Sep 2 2014

ASJC Scopus subject areas

  • Analytical Chemistry


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