Abstract
We demonstrate the first use of smartphone spectrophotometry for readout of fluorescence-based biological assays. We evaluated the smartphone fluorimeter in the context of a fluorescent molecular beacon (MB) assay for detection of specific nucleic acid sequences in a liquid test sample and compared performance against a conventional laboratory fluorimeter. The capability of distinguishing a one-point mismatch is also demonstrated by detecting single-base mutation in target nucleic acids. Our approach offers a route toward portable biomolecular assays for viral/bacterial pathogens, disease biomarkers, and toxins.
Original language | English (US) |
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Pages (from-to) | 8805-8813 |
Number of pages | 9 |
Journal | Analytical Chemistry |
Volume | 86 |
Issue number | 17 |
DOIs | |
State | Published - Sep 2 2014 |
ASJC Scopus subject areas
- Analytical Chemistry