Abstract
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1621-1627 |
| Number of pages | 7 |
| Journal | Lab on a chip |
| Volume | 20 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 7 2020 |
Keywords
- Coronavirus
- COVID-19
- severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
- Novel coronavirus
- Virus
- 2019-nCoV
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- General Chemistry
- Biomedical Engineering
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Respiratory viruses detected in 30 minutes with the help of a smartphone
6/2/20
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