Smart tissue culture: in situ monitoring of the activity of protease enzymes secreted from live cells using nanostructured photonic crystals

Kristopher A. Kilian, Leo M.H. Lai, Astrid Magenau, Sian Cartland, Till Bocking, Nick Di Girolamo, Michael Gal, Katharina Gaus, J. Justin Gooding

Research output: Contribution to journalArticlepeer-review

Abstract

Monitoring enzyme secretion in tissue culture has proved challenging because to date the activity cannot be continuously measured in situ. In this Letter, we present a solution using biopolymer loaded photonic crystals of anodized silicon. Shifts in the optical response by proteolytic degradation of the biopolymer provide label-free sensing with unprecedented low detection limits (1 pg) and calculation of kinetic parameters. The enhancement in sensitivity relative to previous photonic crystal sensors constitutes a change in the sensing paradigm because here the entire pore space is responsive to the secreted enzyme rather than just the pore walls. In situ monitoring is demonstrated by detecting secretion of matrix metalloprotease 9 from stimulated human macrophages.

Original languageEnglish (US)
Pages (from-to)2021-2025
Number of pages5
JournalNano letters
Volume9
Issue number5
DOIs
StatePublished - May 13 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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