TY - JOUR
T1 - Small-Volume Analysis of Cell-Cell Signaling Molecules in the Brain
AU - Romanova, Elena V.
AU - Aerts, Jordan T.
AU - Croushore, Callie A.
AU - Sweedler, Jonathan V.
N1 - Funding Information:
This material is based on work supported by Award Number P30 DA018310 from the National Institute on Drug Abuse, NS031609 from the National Institute of Neurological Disorders and Stroke, and the NSF Division of Chemistry under Grant CHE-11-11705 (with cofunding from the Division of Biological Infrastructure). The content is solely the responsibility of the authors and does not necessarily represent the official views of the award agencies. The remaining authors declare no conflict of interest.
PY - 2014/1
Y1 - 2014/1
N2 - Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and engineering techniques have become useful tools in biological research. The focus of this review is on cutting edge technologies that aid direct measurement of bioactive compounds in the nervous system to facilitate fundamental research, diagnostics, and drug discovery. We discuss challenges associated with measurement of cell-to-cell signaling molecules in the nervous system, and advocate for a decrease of sample volumes to the nanoliter volume regimen for improved analysis outcomes. We highlight effective approaches for the collection, separation, and detection of such small-volume samples, present strategies for targeted and discovery-oriented research, and describe the required technology advances that will empower future translational science.
AB - Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and engineering techniques have become useful tools in biological research. The focus of this review is on cutting edge technologies that aid direct measurement of bioactive compounds in the nervous system to facilitate fundamental research, diagnostics, and drug discovery. We discuss challenges associated with measurement of cell-to-cell signaling molecules in the nervous system, and advocate for a decrease of sample volumes to the nanoliter volume regimen for improved analysis outcomes. We highlight effective approaches for the collection, separation, and detection of such small-volume samples, present strategies for targeted and discovery-oriented research, and describe the required technology advances that will empower future translational science.
KW - CE-MS
KW - lab-on-chip
KW - mass spectrometry
KW - neurotransmitters
KW - sampling
KW - single-cell analysis
UR - http://www.scopus.com/inward/record.url?scp=84890459695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890459695&partnerID=8YFLogxK
U2 - 10.1038/npp.2013.145
DO - 10.1038/npp.2013.145
M3 - Review article
C2 - 23748227
AN - SCOPUS:84890459695
SN - 0893-133X
VL - 39
SP - 50
EP - 64
JO - Neuropsychopharmacology
JF - Neuropsychopharmacology
IS - 1
ER -