Small subunit ribosomal RNA gene phylogeny of Plasmodiophora brassicae

L. A. Castlebury, L. L. Domier

Research output: Contribution to journalArticle

Abstract

The small subunit ribosomal RNA genes of Plasmodiophora brassicae, an obligate endoparasite of crucifers, were amplified using the polymerase chain reaction and found to be approximately 3 kilobases (kb) long. Reverse transcriptase-PCR of the small subunit rRNA produced a fragment approximately 1.8 kb long. Insertion sequences were found at positions 567 (388 bp), 1195 (383 bp), and 1786 (442 bp) of the P. brassicae small subunit ribosomal DNA. Maximum likelihood phylogenetic analysis of the rDNA sequence suggests that P. brassicae may be more closely related to the alveolates (Dinozoa, Ciliophora, and Apicomplexa) than to any of the Fungi, Mycetozoa or Rhizopoda. Bootstrapping (30%), however, does not support this hypothesis, indicating that P. brassicae is not very closely related to any of the organisms in this alignment, including those with which it has been classified in the past.

Original languageEnglish (US)
Pages (from-to)102-107
Number of pages6
JournalMycologia
Volume90
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Clubroot
  • Introns
  • Plasmodiophorales
  • Plasmodiophoromycetes
  • rRNA sequences

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

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