Small RNA-mediated activation of sugar phosphatase mRNA regulates glucose homeostasis

Kai Papenfort, Yan Sun, Masatoshi Miyakoshi, Carin K. Vanderpool, Jörg Vogel

Research output: Contribution to journalArticle

Abstract

Glucose homeostasis is strictly controlled in all domains of life. Bacteria that are unable to balance intracellular sugar levels and deal with potentially toxic phosphosugars cease growth and risk being outcompeted. Here, we identify the conserved haloacid dehalogenase (HAD)-like enzyme YigL as the previously hypothesized phosphatase for detoxification of phosphosugars and reveal that its synthesis is activated by an Hfq-dependent small RNA in Salmonella typhimurium. We show that the glucose-6-P-responsive small RNA SgrS activates YigL synthesis in a translation-independent fashion by the selective stabilization of a decay intermediate of the dicistronic pldB-yigL messenger RNA (mRNA). Intriguingly, the major endoribonuclease RNase E, previously known to function together with small RNAs to degrade mRNA targets, is also essential for this process of mRNA activation. The exploitation of and targeted interference with regular RNA turnover described here may constitute a general route for small RNAs to rapidly activate both coding and noncoding genes.

Original languageEnglish (US)
Pages (from-to)426-437
Number of pages12
JournalCell
Volume153
Issue number2
DOIs
StatePublished - Apr 11 2013

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Homeostasis
Chemical activation
RNA
Glucose
Messenger RNA
Endoribonucleases
Detoxification
Salmonella
Poisons
Salmonella typhimurium
Phosphoric Monoester Hydrolases
Sugars
Bacteria
Stabilization
Genes
sugar-phosphatase
Enzymes
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Small RNA-mediated activation of sugar phosphatase mRNA regulates glucose homeostasis. / Papenfort, Kai; Sun, Yan; Miyakoshi, Masatoshi; Vanderpool, Carin K.; Vogel, Jörg.

In: Cell, Vol. 153, No. 2, 11.04.2013, p. 426-437.

Research output: Contribution to journalArticle

Papenfort, Kai ; Sun, Yan ; Miyakoshi, Masatoshi ; Vanderpool, Carin K. ; Vogel, Jörg. / Small RNA-mediated activation of sugar phosphatase mRNA regulates glucose homeostasis. In: Cell. 2013 ; Vol. 153, No. 2. pp. 426-437.
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