Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning

M. A.M. Spreeuwenberg, J. M.A.J. Verdonk, H Rex Gaskins, M. W.A. Verstegen

Research output: Contribution to journalArticle

Abstract

Compromising alterations in gastrointestinal architecture are common during the weaning transition of pigs. The relation between villous atrophy and epithelial barrier function at weaning is not well understood. This study evaluated in vitro transepithelial transport by Ussing metabolic chambers, local alterations in T-cell subsets and villous architecture at low energy intake level and their relation with lactose/protein ratios in the diet. Pigs (n = 66, 26 d old) were sampled either at weaning (d 0), d 1, 2 or 4 postweaning. Piglets received one of three diets at a low energy intake level, which differed in lactose and protein ratio as follows: low lactose/high protein (LL/HP), control (C), or high lactose/low protein (HL/LP). Mean digestible energy intake was 648 kJ/pig on d 1, 1668 kJ/pig on d 2, 1995 kJ/pig on d 3 and 1990 kJ/pig on d 4 postweaning. The CD4+/CD8+ T-lymphocytes ratio decreased after weaning (P < 0.05). Decreased paracellular transport (P < 0.01), greater villous height (P < 0.01), shallower crypts and lower villus/crypt ratios (P < 0.01) were observed on d 2 compared with d 0. Piglets consuming the HL/LP diet tended to have less paracellular transport (P < 0.10) and greater villous height (P < 0.10) compared with piglets fed the other diets. During the first 4 d postweaning, the effect of diet composition on mucosal integrity was not as important as the sequential effects of low energy intake at weaning. Stress and diminished enteral stimulation seem to compromise mucosal integrity as indicated by increased paracellular transport and altered T-cell subsets.

Original languageEnglish (US)
Pages (from-to)1520-1527
Number of pages8
JournalJournal of Nutrition
Volume131
Issue number5
StatePublished - May 21 2001

Fingerprint

Weaning
Small Intestine
small intestine
Lactose
weaning
Swine
feed intake
lactose
Energy Intake
swine
energy intake
Diet
piglets
T-lymphocytes
T-Lymphocyte Subsets
diet
proteins
Protein-Restricted Diet
Proteins
low protein diet

Keywords

  • Energy intake
  • Epithelial barrier function
  • Piglets
  • T lymphocytes
  • Weaning

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Spreeuwenberg, M. A. M., Verdonk, J. M. A. J., Gaskins, H. R., & Verstegen, M. W. A. (2001). Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. Journal of Nutrition, 131(5), 1520-1527.

Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. / Spreeuwenberg, M. A.M.; Verdonk, J. M.A.J.; Gaskins, H Rex; Verstegen, M. W.A.

In: Journal of Nutrition, Vol. 131, No. 5, 21.05.2001, p. 1520-1527.

Research output: Contribution to journalArticle

Spreeuwenberg, MAM, Verdonk, JMAJ, Gaskins, HR & Verstegen, MWA 2001, 'Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning', Journal of Nutrition, vol. 131, no. 5, pp. 1520-1527.
Spreeuwenberg, M. A.M. ; Verdonk, J. M.A.J. ; Gaskins, H Rex ; Verstegen, M. W.A. / Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. In: Journal of Nutrition. 2001 ; Vol. 131, No. 5. pp. 1520-1527.
@article{3060c502f0b242e1ac8a8a92af0c55a9,
title = "Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning",
abstract = "Compromising alterations in gastrointestinal architecture are common during the weaning transition of pigs. The relation between villous atrophy and epithelial barrier function at weaning is not well understood. This study evaluated in vitro transepithelial transport by Ussing metabolic chambers, local alterations in T-cell subsets and villous architecture at low energy intake level and their relation with lactose/protein ratios in the diet. Pigs (n = 66, 26 d old) were sampled either at weaning (d 0), d 1, 2 or 4 postweaning. Piglets received one of three diets at a low energy intake level, which differed in lactose and protein ratio as follows: low lactose/high protein (LL/HP), control (C), or high lactose/low protein (HL/LP). Mean digestible energy intake was 648 kJ/pig on d 1, 1668 kJ/pig on d 2, 1995 kJ/pig on d 3 and 1990 kJ/pig on d 4 postweaning. The CD4+/CD8+ T-lymphocytes ratio decreased after weaning (P < 0.05). Decreased paracellular transport (P < 0.01), greater villous height (P < 0.01), shallower crypts and lower villus/crypt ratios (P < 0.01) were observed on d 2 compared with d 0. Piglets consuming the HL/LP diet tended to have less paracellular transport (P < 0.10) and greater villous height (P < 0.10) compared with piglets fed the other diets. During the first 4 d postweaning, the effect of diet composition on mucosal integrity was not as important as the sequential effects of low energy intake at weaning. Stress and diminished enteral stimulation seem to compromise mucosal integrity as indicated by increased paracellular transport and altered T-cell subsets.",
keywords = "Energy intake, Epithelial barrier function, Piglets, T lymphocytes, Weaning",
author = "Spreeuwenberg, {M. A.M.} and Verdonk, {J. M.A.J.} and Gaskins, {H Rex} and Verstegen, {M. W.A.}",
year = "2001",
month = "5",
day = "21",
language = "English (US)",
volume = "131",
pages = "1520--1527",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "5",

}

TY - JOUR

T1 - Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning

AU - Spreeuwenberg, M. A.M.

AU - Verdonk, J. M.A.J.

AU - Gaskins, H Rex

AU - Verstegen, M. W.A.

PY - 2001/5/21

Y1 - 2001/5/21

N2 - Compromising alterations in gastrointestinal architecture are common during the weaning transition of pigs. The relation between villous atrophy and epithelial barrier function at weaning is not well understood. This study evaluated in vitro transepithelial transport by Ussing metabolic chambers, local alterations in T-cell subsets and villous architecture at low energy intake level and their relation with lactose/protein ratios in the diet. Pigs (n = 66, 26 d old) were sampled either at weaning (d 0), d 1, 2 or 4 postweaning. Piglets received one of three diets at a low energy intake level, which differed in lactose and protein ratio as follows: low lactose/high protein (LL/HP), control (C), or high lactose/low protein (HL/LP). Mean digestible energy intake was 648 kJ/pig on d 1, 1668 kJ/pig on d 2, 1995 kJ/pig on d 3 and 1990 kJ/pig on d 4 postweaning. The CD4+/CD8+ T-lymphocytes ratio decreased after weaning (P < 0.05). Decreased paracellular transport (P < 0.01), greater villous height (P < 0.01), shallower crypts and lower villus/crypt ratios (P < 0.01) were observed on d 2 compared with d 0. Piglets consuming the HL/LP diet tended to have less paracellular transport (P < 0.10) and greater villous height (P < 0.10) compared with piglets fed the other diets. During the first 4 d postweaning, the effect of diet composition on mucosal integrity was not as important as the sequential effects of low energy intake at weaning. Stress and diminished enteral stimulation seem to compromise mucosal integrity as indicated by increased paracellular transport and altered T-cell subsets.

AB - Compromising alterations in gastrointestinal architecture are common during the weaning transition of pigs. The relation between villous atrophy and epithelial barrier function at weaning is not well understood. This study evaluated in vitro transepithelial transport by Ussing metabolic chambers, local alterations in T-cell subsets and villous architecture at low energy intake level and their relation with lactose/protein ratios in the diet. Pigs (n = 66, 26 d old) were sampled either at weaning (d 0), d 1, 2 or 4 postweaning. Piglets received one of three diets at a low energy intake level, which differed in lactose and protein ratio as follows: low lactose/high protein (LL/HP), control (C), or high lactose/low protein (HL/LP). Mean digestible energy intake was 648 kJ/pig on d 1, 1668 kJ/pig on d 2, 1995 kJ/pig on d 3 and 1990 kJ/pig on d 4 postweaning. The CD4+/CD8+ T-lymphocytes ratio decreased after weaning (P < 0.05). Decreased paracellular transport (P < 0.01), greater villous height (P < 0.01), shallower crypts and lower villus/crypt ratios (P < 0.01) were observed on d 2 compared with d 0. Piglets consuming the HL/LP diet tended to have less paracellular transport (P < 0.10) and greater villous height (P < 0.10) compared with piglets fed the other diets. During the first 4 d postweaning, the effect of diet composition on mucosal integrity was not as important as the sequential effects of low energy intake at weaning. Stress and diminished enteral stimulation seem to compromise mucosal integrity as indicated by increased paracellular transport and altered T-cell subsets.

KW - Energy intake

KW - Epithelial barrier function

KW - Piglets

KW - T lymphocytes

KW - Weaning

UR - http://www.scopus.com/inward/record.url?scp=0035004641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035004641&partnerID=8YFLogxK

M3 - Article

C2 - 11340110

AN - SCOPUS:0035004641

VL - 131

SP - 1520

EP - 1527

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 5

ER -