Small Heterodimer Partner Regulates Dichotomous T Cell Expansion by Macrophages

Sayyed Hamed Shahoei, Young Chae Kim, Samuel J. Cler, Liqian Ma, Sayeepriyadarshini Anakk, Jongsook K. Kemper, Erik R. Nelson

Research output: Contribution to journalArticle

Abstract

The involvement of small heterodimer partner (SHP) in the inhibition of hepatic bile acid synthesis from cholesterol has been established. However, extrahepatic expression of SHP implies that SHP may have regulatory functions other than those in the liver. Here, we find that SHP mRNA expression is high in murine bone marrow cells, suggesting a physiological role within macrophages. Indeed, expression of SHP in macrophages decreases the transcriptional activity and nuclear localization of nuclear factor κB, whereas downregulation of SHP has the opposite effects. Expression of genes associated with macrophage-T cell crosstalk were altered by overexpression or downregulation of SHP. Intriguingly, increasing SHP expression in macrophages resulted in decreased T cell expansion, a hallmark of T cell activation, whereas knockdown of SHP resulted in increased expansion. Analyses of the expanded T cells revealed a dichotomous skewing between effector T cells and regulatory T cells (Tregs), with SHP overexpression reducing Tregs and downregulation of SHP increasing their expansion. The expanded Tregs were confirmed to be suppressive via adoptive transfers. IL-2 and TGF-β, known inducers of Treg differentiation, were found to be regulated by SHP. Furthermore, SHP occupancy at the promoter region of IL-2 was increased after macrophages were challenged with lipopolysaccharide. Neutralizing antibodies to IL-2 and TGF-β inhibited the expansion of Tregs mediated by downregulation of SHP. This study demonstrates that expression and activity of SHP within macrophages can alter T cell fate and identifies SHP as a potential therapeutic target for autoimmune diseases or solid cancers.

Original languageEnglish (US)
Pages (from-to)1573-1589
Number of pages17
JournalEndocrinology
Volume160
Issue number7
DOIs
StatePublished - Jan 1 2019

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Macrophages
T-Lymphocytes
Down-Regulation
Interleukin-2
Adoptive Transfer
Liver
Regulatory T-Lymphocytes
Neutralizing Antibodies
Bile Acids and Salts
Genetic Promoter Regions
Bone Marrow Cells
Autoimmune Diseases
Lipopolysaccharides
Cholesterol
Gene Expression
Messenger RNA
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Endocrinology

Cite this

Small Heterodimer Partner Regulates Dichotomous T Cell Expansion by Macrophages. / Shahoei, Sayyed Hamed; Kim, Young Chae; Cler, Samuel J.; Ma, Liqian; Anakk, Sayeepriyadarshini; Kemper, Jongsook K.; Nelson, Erik R.

In: Endocrinology, Vol. 160, No. 7, 01.01.2019, p. 1573-1589.

Research output: Contribution to journalArticle

Shahoei, Sayyed Hamed ; Kim, Young Chae ; Cler, Samuel J. ; Ma, Liqian ; Anakk, Sayeepriyadarshini ; Kemper, Jongsook K. ; Nelson, Erik R. / Small Heterodimer Partner Regulates Dichotomous T Cell Expansion by Macrophages. In: Endocrinology. 2019 ; Vol. 160, No. 7. pp. 1573-1589.
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