Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate, but do not affect Rubisco carboxylation capacity

Elizabeth P. Harrison, Hulya Olcer, Julie C. Lloyd, Stephen P. Long, Christine A. Raines

Research output: Contribution to journalArticle

Abstract

The response of net photosynthetic CO2 uptake (A) to increasing leaf intercellular CO2 concentration (ci) was determined in antisense Nicotiana tabacum plants, derived from six independent transformation lines, displaying a range of sedoheptulose-1, 7-bisphosphatase (SBPase) activities. The maximum in vivo ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) carboxylation (Vc,max) and RuBP regeneration (Jmax) rates were calculated from the steady-state measurements of the A to ci response curves. In plants with reductions in SBPase activity of between 9% and 60%, maximum RuBP regeneration capacity declined linearly (r2 = 0.79) and no significant change in apparent in vivo Rubisco activity (Vc.max) was observed in these plants. No correlation between Vc,max and a decrease in capacity for RuBP regeneration was observed (r2=0.14) in the SBPase antisense plants. These data demonstrate that small decreases in SBPase activity limit photosynthetic carbon assimilation by reducing the capacity for RuBP regeneration.

Original languageEnglish (US)
Pages (from-to)1779-1784
Number of pages6
JournalJournal of experimental botany
Volume52
Issue number362
DOIs
StatePublished - Jan 1 2001

Fingerprint

Oxygenases
carboxylation
ribulose 1,5-diphosphate
oxygenases
Regeneration
carbon dioxide
Nicotiana tabacum
Tobacco
Carbon
uptake mechanisms
sedoheptulose
ribulose-1,5 diphosphate
leaves

Keywords

  • Antisense
  • Photosynthesis
  • Rubisco
  • SBPase

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate, but do not affect Rubisco carboxylation capacity. / Harrison, Elizabeth P.; Olcer, Hulya; Lloyd, Julie C.; Long, Stephen P.; Raines, Christine A.

In: Journal of experimental botany, Vol. 52, No. 362, 01.01.2001, p. 1779-1784.

Research output: Contribution to journalArticle

Harrison, Elizabeth P. ; Olcer, Hulya ; Lloyd, Julie C. ; Long, Stephen P. ; Raines, Christine A. / Small decreases in SBPase cause a linear decline in the apparent RuBP regeneration rate, but do not affect Rubisco carboxylation capacity. In: Journal of experimental botany. 2001 ; Vol. 52, No. 362. pp. 1779-1784.
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