TY - JOUR
T1 - Slide-free virtual histochemistry (Part II)
T2 - Detection of field cancerization
AU - You, Sixian
AU - Sun, Yi
AU - Chaney, Eric J.
AU - Zhao, Youbo
AU - Chen, Jianxin
AU - Boppart, Stephen A.
AU - Tu, Haohua
N1 - Funding Information:
National Cancer Institute (R01CA166309 and R01CA213149); National Institute of Biomedical Imaging and Bioengineering (R01EB023232) of the National Institutes of Health.
Publisher Copyright:
© 2018 Optical Society of America.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Tumor-adjacent “normal” tissue constitutes a peri-tumoral field that affects early cancer detection, risk assessment, surgical decision, and postoperative surveillance. Modern genetic analysis has revealed valuable information from this field, but without the spatial resolution of optical microscopy to understand the vital microenvironments that surround individual cells. Rapidly advanced optical imaging techniques free of labor-intensive sample preparation, despite great promise to perform slide-free imaging of cell structure and shift the histology-centered cancer diagnostic paradigm, have lacked compatible and complementary histochemical imaging of cell function or phenotype to interrogate the peri-tumoral field. In the first part (Part I) of this two-part series study, we developed a technique of slide-free virtual histochemistry to phenotype various cells in in vivo animal and ex vivo human tissue. Here, in the second part (Part II) of this two-part series study, we employ this technique to examine various peri-tumoral fields and produce the volumetric histochemical evidence of field cancerization consistent with the structural changes at larger spatial scales. We also link the field cancerization with cancer dormancy in a significant portion of breast cancer patients.
AB - Tumor-adjacent “normal” tissue constitutes a peri-tumoral field that affects early cancer detection, risk assessment, surgical decision, and postoperative surveillance. Modern genetic analysis has revealed valuable information from this field, but without the spatial resolution of optical microscopy to understand the vital microenvironments that surround individual cells. Rapidly advanced optical imaging techniques free of labor-intensive sample preparation, despite great promise to perform slide-free imaging of cell structure and shift the histology-centered cancer diagnostic paradigm, have lacked compatible and complementary histochemical imaging of cell function or phenotype to interrogate the peri-tumoral field. In the first part (Part I) of this two-part series study, we developed a technique of slide-free virtual histochemistry to phenotype various cells in in vivo animal and ex vivo human tissue. Here, in the second part (Part II) of this two-part series study, we employ this technique to examine various peri-tumoral fields and produce the volumetric histochemical evidence of field cancerization consistent with the structural changes at larger spatial scales. We also link the field cancerization with cancer dormancy in a significant portion of breast cancer patients.
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U2 - 10.1364/BOE.9.005253
DO - 10.1364/BOE.9.005253
M3 - Article
C2 - 30460126
AN - SCOPUS:85056600888
VL - 9
SP - 5253
EP - 5268
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 11
M1 - #332413
ER -