TY - JOUR
T1 - Slide-free virtual histochemistry (Part I)
T2 - Development via nonlinear optics
AU - You, Sixian
AU - Sun, Y. I.
AU - Chaney, Eric J.
AU - Zhao, Youbo
AU - Chen, Jianxin
AU - Boppart, Stephen A.
AU - Tu, Haohua
N1 - National Cancer Institute (R01CA166309 and R01CA213149); National Institute of Biomedical Imaging and Bioengineering (R01EB023232) of the National Institutes of Health.We thank Darold Spillman for his general technical support, Marina Marjanovic for securing human tissue specimens and obtaining relevant pathological reports, Art Camire and Tommi Hakulinen from Spectra-Physics for their support and the use of a 10 MHz Yb-based laser (femtoTrain), and Cecile Mukerjee and Yoann Zaouter at Amplitude Systemes for kindly supplying the fiber laser (Satsuma) to optimize the fiber supercontinuum source. We thank Anna Higham, Kimberly Cradock, Natasha Luckey, and Z. George Liu from Carle Foundation Hospital for assisting in the acquisition and histological assessment of the human tissue specimens in this study. We also thank the Carle Research Office for assisting in the coordination and consenting in this human subjects research.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments in label-free optical imaging has demonstrated the potential to replace the conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse optical interactions to generate histochemical contrasts, allowing “virtual” histochemistry in three spatial dimensions without preparing a microscope slide (i.e. labor-intensive sample preparation). However, the histochemical information in a label-free optical image has often been rather limited due to the difficulty in simultaneously generating multiple histochemical contrasts with strict spatial co-registration. Here, in the first part (Part I) of this two-part series study, we develop a technique of slide-free virtual histochemistry based on label-free multimodal multiphoton microscopy, and simultaneously generate up to four histochemical contrasts from in vivo animal and ex vivo human tissue. To enable this functionality, we construct and demonstrate a robust fiber-based laser source for clinical translation and phenotype a wide variety of vital cells in unperturbed mammary tissue.
AB - Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments in label-free optical imaging has demonstrated the potential to replace the conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse optical interactions to generate histochemical contrasts, allowing “virtual” histochemistry in three spatial dimensions without preparing a microscope slide (i.e. labor-intensive sample preparation). However, the histochemical information in a label-free optical image has often been rather limited due to the difficulty in simultaneously generating multiple histochemical contrasts with strict spatial co-registration. Here, in the first part (Part I) of this two-part series study, we develop a technique of slide-free virtual histochemistry based on label-free multimodal multiphoton microscopy, and simultaneously generate up to four histochemical contrasts from in vivo animal and ex vivo human tissue. To enable this functionality, we construct and demonstrate a robust fiber-based laser source for clinical translation and phenotype a wide variety of vital cells in unperturbed mammary tissue.
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U2 - 10.1364/BOE.9.005240
DO - 10.1364/BOE.9.005240
M3 - Article
C2 - 30460125
AN - SCOPUS:85056568839
SN - 2156-7085
VL - 9
SP - 5240
EP - 5252
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
M1 - #332416
ER -