Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli

D. S. Froese, C. M. Dobson, A. P. White, X. Wu, D. Padovani, R. Banerjee, T. Haller, John Alan Gerlt, M. G. Surette, R. A. Gravel

Research output: Contribution to journalArticle

Abstract

In Escherichia coli, a four-gene operon, sbm-ygfD-ygfG-ygfH, has been shown to encode a putative cobalamin-dependent pathway with the ability to produce propionate from succinate in vitro [Haller T, Buckel T, Retey J, Gerlt JA. Discovering new enzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli. Biochemistry 2000;39:4622-4629]. However, the operon was thought to be silent in vivo, illustrated by the eponym describing its first gene, "sleeping beauty mutase" (methylmalonyl-CoA mutase, MCM). Of the four genes described, only ygfD could not be assigned a function. In this study, we have evaluated the functional integrity of YgfD and Sbm and show that, indeed, both proteins are expressed in E. coli and that YgfD has GTPase activity. We show that YgfD and Sbm can be co-immunoprecipitated from E. coli extracts using antibody to either protein, demonstrating in vivo interaction, a result confirmed using a strain deleted for ygfD. We show further that, in vitro, purified His-tagged YgfD and Sbm behave as a monomer and dimer, respectively, and that they form a multi-subunit complex that is dependent on pre-incubation of YgfD with non-hydrolysable GTP, an outcome that was not affected by the state of Sbm, as holo- or apoenzyme. These studies reinforce a role for the in vivo interaction of YgfD and Sbm.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalMicrobiological Research
Volume164
Issue number1
DOIs
StatePublished - Jan 1 2009

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Intramolecular Transferases
Beauty
Escherichia coli
Propionates
Succinic Acid
Operon
Methylmalonyl-CoA Mutase
Eponyms
Genes
Apoenzymes
Holoenzymes
Aptitude
GTP Phosphohydrolases
Vitamin B 12
Guanosine Triphosphate
Metabolic Networks and Pathways
Biochemistry
Proteins
Antibodies
Enzymes

Keywords

  • Cobalamin
  • Methylmalonyl-CoA mutase
  • Sbm
  • Vitamin B
  • YgfD

ASJC Scopus subject areas

  • Microbiology

Cite this

Froese, D. S., Dobson, C. M., White, A. P., Wu, X., Padovani, D., Banerjee, R., ... Gravel, R. A. (2009). Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli. Microbiological Research, 164(1), 1-8. https://doi.org/10.1016/j.micres.2008.08.006

Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli. / Froese, D. S.; Dobson, C. M.; White, A. P.; Wu, X.; Padovani, D.; Banerjee, R.; Haller, T.; Gerlt, John Alan; Surette, M. G.; Gravel, R. A.

In: Microbiological Research, Vol. 164, No. 1, 01.01.2009, p. 1-8.

Research output: Contribution to journalArticle

Froese, DS, Dobson, CM, White, AP, Wu, X, Padovani, D, Banerjee, R, Haller, T, Gerlt, JA, Surette, MG & Gravel, RA 2009, 'Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli', Microbiological Research, vol. 164, no. 1, pp. 1-8. https://doi.org/10.1016/j.micres.2008.08.006
Froese, D. S. ; Dobson, C. M. ; White, A. P. ; Wu, X. ; Padovani, D. ; Banerjee, R. ; Haller, T. ; Gerlt, John Alan ; Surette, M. G. ; Gravel, R. A. / Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli. In: Microbiological Research. 2009 ; Vol. 164, No. 1. pp. 1-8.
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