Skeletal muscle and liver gene expression profiles in finishing steers supplemented with Amaize

Ahmed A. Elolimy, Sonia J. Moisá, Kristen M. Brennan, Allison C. Smith, Daniel Graugnard, Daniel W. Shike, Juan J. Loor

Research output: Contribution to journalArticlepeer-review


Our main objective was to evaluate the effects of feeding α-amylase (Amaize, Alltech Inc., Nicholasville, KY, USA) for 140 days on skeletal muscle and liver gene transcription in beef steers. Steers fed Amaize had lower average daily gain (p =.03) and gain:feed ratio (p =.05). No differences (p >.10) in serum metabolites or carcass traits were detected between the two groups but Amaize steers tended (p <.15) to have increased 12th rib fat depth. Microarray analysis of skeletal muscle revealed 21 differentially expressed genes (DEG), where 14 were up-regulated and seven were down-regulated in Amaize-fed steers. The bioinformatics analysis indicated that metabolic pathways involved in fat formation and deposition, stress response, and muscle function were activated, while myogenesis was inhibited in Amaize-fed steers. The quantitative PCR results for liver revealed a decrease (p <.01) in expression of fatty acid binding protein 1 (FABP1) and 3-hydroxybutyrate dehydrogenase 1 (BDH1) with Amaize. Because these genes are key for intracellular fatty acid transport, oxidation and ketone body production, data suggest a reduction in hepatic lipid catabolism. Future work to investigate potential positive effects of Amaize on cellular stress response, muscle function, and liver function in beef cattle appears warranted.

Original languageEnglish (US)
Pages (from-to)1107-1119
Number of pages13
JournalAnimal Science Journal
Issue number8
StatePublished - Aug 2018


  • bioinformatics
  • gene expression
  • transcriptomics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)


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