Site-specific 13C labeling of DNA to deduce DNA repair mechanisms of uracil-DNA glycosylase and UV endonuclease V

M. Manoharan, A. Mazumder, J. A. Gerlt

Research output: Contribution to journalArticlepeer-review

Abstract

The oligodeoxynucleotide d(GCGUGCG) was synthesized with [l', 3'-13C2)U labeling. The uracil unit was removed with uracil-DNA glycosylase to generate an abasic site and the resulting oligonucleotide was paired with the possible d(CGCNCGC) structures. One of these heteroduplexes was a substrate for UV endonuclease V. The 13C NMR spectra of these heteroduplexes describe the structure of the abasic site and the mechanism of the endonuclease reaction.

Original languageEnglish (US)
Pages (from-to)879-883
Number of pages5
JournalNucleosides and Nucleotides
Volume8
Issue number5-6
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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