Site-specific attachment of proteins onto a 3D DNA tetrahedron through backbone-modified phosphorothioate DNA

Ngo Yin Wong, Chuan Zhang, Li Huey Tan, Yi Lu

Research output: Contribution to journalArticle

Abstract

Phosphorothioate-modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and the modification can be programmably placed within the structure.

Original languageEnglish (US)
Pages (from-to)1427-1430
Number of pages4
JournalSmall
Volume7
Issue number10
DOIs
StatePublished - May 23 2011

Keywords

  • DNA structures
  • nanostructures
  • phosphorothioate
  • proteins
  • self-assembly

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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