Single-step fabrication and characterization of photonic crystal biosensors with polymer microfluidic channels

Charles J. Choi, Brian T. Cunningham

Research output: Contribution to journalArticle

Abstract

A method for simultaneously integrating label-free photonic crystal biosensor technology into microfluidic channels by a single-step replica molding process is presented. By fabricating both the sub-micron features of the photonic crystal sensor structure and the >10 μm features of a flow channel network in one step at room temperature on a plastic substrate, the sensors are automatically self-aligned with the flow channels, and patterns of arbitrary shape may be produced. By measuring changes in the resonant peak reflected wavelength from the photonic crystal structure induced by changes in dielectric permittivity within an evanescent field region near its surface, detection of bulk refractive index changes in the fluid channel or adsorption of biological material to the sensor surface is demonstrated. An imaging detection instrument is used to characterize the spatial distribution of the photonic crystal resonant wavelength, gathering thousands of independent sensor readings within a single fluid channel.

Original languageEnglish (US)
Pages (from-to)1373-1380
Number of pages8
JournalLab on a chip
Volume6
Issue number10
DOIs
StatePublished - 2006

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Single-step fabrication and characterization of photonic crystal biosensors with polymer microfluidic channels'. Together they form a unique fingerprint.

  • Cite this