Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

Till Böcking; Kristopher A. Kilian; Katharina Gaus; J. Justin Gooding

doi:10.1021/la060331a

Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

Till Böcking, Kristopher A. Kilian, Katharina Gaus, J. Justin Gooding

Research output: Contribution to journal › Article › peer-review

Abstract

Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.

Original language	English (US)
Pages (from-to)	3494-3496
Number of pages	3
Journal	Langmuir
Volume	22
Issue number	8
DOIs	https://doi.org/10.1021/la060331a
State	Published - Apr 11 2006
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la060331a

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AU - Böcking, Till

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AU - Gooding, J. Justin

PY - 2006/4/11

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AB - Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.

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Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

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