Single-particle tracking reveals a dynamic role of actin filaments in assisting long-range axonal transport in neurons

Yasuko Osakada, Kai Zhang

Research output: Contribution to journalArticle

Abstract

Here, we demonstrated that actin filaments mediate axonal transport in dorsal root ganglia (DRG) neurons using fluorescence single-particle tracking. We employed a compartmentalized microfluidic cell culturing chamber that allows depolymerization of actin filaments within an axonal segment. We observed that local actin depolymerization results in a two-fold increase in the average pausing duration, whereas the microtubuledependent instantaneous transport speed is not perturbed. Collectively, our data reveal an important role of actin filaments in assisting microtubule-dependent long-range NGF axonal transport in DRG neurons.

Original languageEnglish (US)
Pages (from-to)714-719
Number of pages6
JournalBulletin of the Chemical Society of Japan
Volume90
Issue number6
DOIs
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Chemistry(all)

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