Here, we demonstrated that actin filaments mediate axonal transport in dorsal root ganglia (DRG) neurons using fluorescence single-particle tracking. We employed a compartmentalized microfluidic cell culturing chamber that allows depolymerization of actin filaments within an axonal segment. We observed that local actin depolymerization results in a two-fold increase in the average pausing duration, whereas the microtubuledependent instantaneous transport speed is not perturbed. Collectively, our data reveal an important role of actin filaments in assisting microtubule-dependent long-range NGF axonal transport in DRG neurons.
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