Single Particle Tracking: From Theory to Biophysical Applications

Hao Shen, Lawrence J. Tauzin, Rashad Baiyasi, Wenxiao Wang, Nicholas Moringo, Bo Shuang, Christy F. Landes

Research output: Contribution to journalReview articlepeer-review


After three decades of developments, single particle tracking (SPT) has become a powerful tool to interrogate dynamics in a range of materials including live cells and novel catalytic supports because of its ability to reveal dynamics in the structure-function relationships underlying the heterogeneous nature of such systems. In this review, we summarize the algorithms behind, and practical applications of, SPT. We first cover the theoretical background including particle identification, localization, and trajectory reconstruction. General instrumentation and recent developments to achieve two- and three-dimensional subdiffraction localization and SPT are discussed. We then highlight some applications of SPT to study various biological and synthetic materials systems. Finally, we provide our perspective regarding several directions for future advancements in the theory and application of SPT.

Original languageEnglish (US)
Pages (from-to)7331-7376
Number of pages46
JournalChemical reviews
Issue number11
StatePublished - Jun 14 2017
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry


Dive into the research topics of 'Single Particle Tracking: From Theory to Biophysical Applications'. Together they form a unique fingerprint.

Cite this