Abstract
Styrene-maleic acid (SMA) copolymers can extract membrane proteins from native membranes along with lipids as nanodiscs. Preparation with SMA is fast, cost-effective, and captures the native protein-lipid interactions. On the other hand, cryo-EM has become increasingly successful and efficient for structural determinations of membrane proteins, with biochemical sample preparation often the bottleneck. Three recent cryo-EM studies on the efflux transporter AcrB and the alternative complex III: cyt c oxidase supercomplex have demonstrated the potential of SMA nanodisc samples to yield high-resolution structure information of membrane proteins.
Original language | English (US) |
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Pages (from-to) | 114-119 |
Number of pages | 6 |
Journal | Chemistry and Physics of Lipids |
Volume | 221 |
DOIs | |
State | Published - Jul 2019 |
Keywords
- Alternative complex III
- Cryo-EM
- Lipid-protein interaction
- Membrane protein
- Multidrug transporter AcrB
- Nanodisc
- SMA
- Styrene-maleic acid copolymer
- Triacylated cysteine
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Cell Biology