Abstract

A technique to identify and quantitate simultaneously more than 30 compounds in individual neurons is described. The method uses nanoliter volume sampling, capillary electrophoresis separation, and wavelength- resolved native fluorescence detection. Limits of detection (LODs) range from the low attomole to the femtomole rungs, with 5-hydroxytryptamine (or serotonin [5-HT]) LODs being ~20 attomoles. Although the cellular sample matrix is chemically complex, the combination of electrophoretic migration time and fluorescence spectral information allows positive identification of aromatic monoamines, aromatic amino acids and peptides containing them, flavins, adenosine-and guanosine-nucleotide analogs, and other fluorescent compounds. Individual identified neurons from Aplysia californica and Pleurobranchaea californica are used to demonstrate the applicability and figures of merit of this technique.

Original languageEnglish (US)
Pages (from-to)173-181
Number of pages9
JournalNeuron
Volume20
Issue number2
DOIs
StatePublished - Feb 1998

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Fluorescence Spectrometry
Capillary Electrophoresis
Pleurobranchaea
Limit of Detection
Serotonin
Fluorescence
Flavins
Neurons
Aplysia
Aromatic Amino Acids
Guanosine
Adenosine
Nucleotides
Peptides
compound 30

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Single neuron analysis by capillary electrophoresis with fluorescence spectroscopy. / Fuller, Robert R.; Moroz, Leonid L.; Gillette, Rhanor; Sweedler, Jonathan V.

In: Neuron, Vol. 20, No. 2, 02.1998, p. 173-181.

Research output: Contribution to journalArticle

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