Abstract

We demonstrate a label-free biosensor imaging approach that utilizes a photonic crystal (PC) surface to detect surface attachment of individual dielectric and metal nanoparticles through measurement of localized shifts in the resonant wavelength and resonant reflection magnitude from the PC. Using a microscopy-based approach to scan the PC resonant reflection properties with 0.6 μm spatial resolution, we show that metal nanoparticles attached to the biosensor surface with strong absorption at the resonant wavelength induce a highly localized reduction in reflection efficiency and are able to be detected by modulation of the resonant wavelength. Experimental demonstrations of single-nanoparticle imaging are supported by finite-difference time-domain computer simulations. The ability to image surface-adsorption of individual nanoparticles offers a route to single molecule biosensing, in which the particles can be functionalized with specific recognition molecules and utilized as tags. This journal is

Original languageEnglish (US)
Pages (from-to)1007-1015
Number of pages9
JournalAnalyst
Volume139
Issue number5
DOIs
StatePublished - Feb 3 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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  • Cite this

    Zhuo, Y., Hu, H., Chen, W., Lu, M., Tian, L., Yu, H., Long, K. D., Chow, E., King, W. P., Singamaneni, S., & Cunningham, B. T. (2014). Single nanoparticle detection using photonic crystal enhanced microscopy. Analyst, 139(5), 1007-1015. https://doi.org/10.1039/c3an02295a