Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA Templates

Research output: Contribution to journalArticle

Abstract

HIV-1 RT (human immunodeficiency virus-1 reverse transcriptase) is a multifunctional polymerase responsible for reverse transcription of the HIV genome, including DNA replication on both RNA and DNA templates. During reverse transcription in vivo, HIV-1 RT replicates through various secondary structures on RNA and single-stranded DNA (ssDNA) templates without the need for a nucleic acid unwinding protein, such as a helicase. In order to understand the mechanism of polymerization through secondary structures, we investigated the DNA polymerization activity of HIV-1 RT on long ssDNA templates using a multiplexed single-molecule DNA flow-stretching assay. We observed that HIV-1 RT performs fast primer extension DNA synthesis on single-stranded regions of DNA (18.7 nt/s) and switches its activity to slow strand displacement synthesis at DNA hairpin locations (2.3 nt/s). Furthermore, we found that the rate of strand displacement synthesis is dependent on the GC content in hairpin stems and template stretching force. This indicates that the strand displacement synthesis occurs through a mechanism that is neither completely active nor passive: that is, the opening of the DNA hairpin is driven by a combination of free energy released during dNTP (deoxyribonucleotide triphosphate) hydrolysis and thermal fraying of base pairs. Our experimental observations provide new insight into the interchanging modes of DNA replication by HIV-1 RT on long ssDNA templates.

Original languageEnglish (US)
Pages (from-to)995-1006
Number of pages12
JournalJournal of Molecular Biology
Volume395
Issue number5
DOIs
StatePublished - Feb 5 2010

Fingerprint

Polymerization
Single-Stranded DNA
HIV-1
DNA
DNA Replication
Reverse Transcription
RNA
Deoxyribonucleotides
DNA Primers
Base Composition
Base Pairing
Nucleic Acids
Hydrolysis
Hot Temperature
Human immunodeficiency virus 1 reverse transcriptase
HIV
Genome
Proteins

Keywords

  • DNA flow-stretching assay
  • DNA hairpin
  • HIV-1 reverse transcriptase
  • single molecule
  • strand displacement synthesis

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA Templates. / Kim, Sangjin; Schroeder, Charles M.; Xie, X. Sunney.

In: Journal of Molecular Biology, Vol. 395, No. 5, 05.02.2010, p. 995-1006.

Research output: Contribution to journalArticle

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