Single molecule Raman spectroscopic assay to detect transgene from GM plants

Ulhas S. Kadam, Rahul L. Chavhan, Burkhard Schulz, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Substantial concerns have been raised for the safety of transgenics on human health and environment. Many organizations, consumer groups, and environmental agencies advocate for stringent regulations to avoid transgene products' contamination in food cycle or in nature. Here we demonstrate a novel approach using surface enhanced Raman spectroscopy (SERS) to detect and quantify transgene from GM plants. We show a highly sensitive and accurate quantification of transgene DNA from multiple transgenic lines of Arabidopsis. The assay allows us to detect and quantify the transgenes as low as 0.10 pg without need for PCR-amplification. This technology is relatively cheap, quick, simple, and suitable for detection at low target concentration.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalAnalytical Biochemistry
Volume532
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

Keywords

  • Biosensors
  • CaMV-35S
  • Genetically modified plants (GMOs)
  • NPTII gene
  • Surface-enhanced Raman spectroscopy (SERS)
  • T-DNA
  • Transgene detection

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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