Single-molecule quantum-dot fluorescence resonance energy transfer

Sungchul Hohng, Taekjip Ha

Research output: Contribution to journalArticlepeer-review


Colloidal semiconductor quantum dots are promising for single-molecule biological Imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic flurophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules can be observed by using the quantum dot as the donor.

Original languageEnglish (US)
Pages (from-to)956-960
Number of pages5
Issue number5
StatePublished - May 2005


  • DNA
  • FRET
  • Fluorescence
  • Quantum dots
  • Single-molecule studies

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry


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