Single-molecule methods in polymer science

Steve Granick, Sung Chul Bae, Bo Wang, Subhalakshmi Kumar, Juan Guan, Changqian Yu, Kejia Chen, James Kuo

Research output: Contribution to journalArticle


The single-molecule fluorescence methods offer several advantages to the biophysics researchers. One of the advantages is that the fluorescence measurements can be performed in situ, though it remains true that the spatial resolution of electron microscopy is better. The use of a single-molecule tracking to follow the Gaussian center of a blurry diffraction-limited image, it may be a few nanometers to a few tens of nanometers. Conformational mobility and transport can also be studied. Tracking the transport of individual polymer molecules finds wider application when the moving molecules become larger, large enough that the molecule exceeds the spatial resolution. The technique of fluorescence correlation spectroscopy averages over the course of time over many molecules, each of them measured with single-molecule sensitivity for a short time, allowing single-polymer diffusion to be measured at surfaces and in ultra-dilute solutions.

Original languageEnglish (US)
Pages (from-to)2542-2543
Number of pages2
JournalJournal of Polymer Science, Part B: Polymer Physics
Issue number24
StatePublished - Dec 15 2010


  • diffusion
  • ensemble average
  • fluorescence
  • imaging
  • nanotechnology
  • optics
  • single molecule
  • transport

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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  • Cite this

    Granick, S., Bae, S. C., Wang, B., Kumar, S., Guan, J., Yu, C., Chen, K., & Kuo, J. (2010). Single-molecule methods in polymer science. Journal of Polymer Science, Part B: Polymer Physics, 48(24), 2542-2543.