Abstract

This work describes atomic force microscopy studies of the physical parameters determining the strength of lipid anchorage in bilayers as a function of the phase state of the lipid matrix. These investigations used biotinylated lipids and streptavidin-derivatized tips to quantify the lipid pullout force from different lipid matrices. Analysis of the data using both dynamic force spectroscopy and full microscopic models show that the anchorage strength is greater in gel-phase relative to fluid-phase lipids. Additional model parameter estimates provide further insights into the hidden energy barriers that determine the mechanical integrity of lipid anchors in biological membranes.

Original languageEnglish (US)
Pages (from-to)5985-5993
Number of pages9
JournalJournal of Physical Chemistry B
Volume109
Issue number12
DOIs
StatePublished - Mar 31 2005

Fingerprint

Phase behavior
Anchors
Lipids
lipids
Molecules
molecules
Biological membranes
Streptavidin
Atomic Force Microscopy
Energy barriers
matrices
integrity
Atomic force microscopy
Spectrum Analysis
Gels
atomic force microscopy
Spectroscopy
gels
membranes
Fluids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Single-molecule measurements of the impact of lipid phase behavior on anchor strengths. / Wieland, Julie A.; Gewirth, Andrew A.; Leckband, Deborah E.

In: Journal of Physical Chemistry B, Vol. 109, No. 12, 31.03.2005, p. 5985-5993.

Research output: Contribution to journalArticle

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