Single-molecule measurements of the impact of lipid phase behavior on anchor strengths

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This work describes atomic force microscopy studies of the physical parameters determining the strength of lipid anchorage in bilayers as a function of the phase state of the lipid matrix. These investigations used biotinylated lipids and streptavidin-derivatized tips to quantify the lipid pullout force from different lipid matrices. Analysis of the data using both dynamic force spectroscopy and full microscopic models show that the anchorage strength is greater in gel-phase relative to fluid-phase lipids. Additional model parameter estimates provide further insights into the hidden energy barriers that determine the mechanical integrity of lipid anchors in biological membranes.

Original languageEnglish (US)
Pages (from-to)5985-5993
Number of pages9
JournalJournal of Physical Chemistry B
Issue number12
StatePublished - Mar 31 2005

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry


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