Single-molecule FRET analysis of helicase functions

Eli Rothenberg, Taekjip Ha

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In recent years, advancements in single-biomolecule probing techniques have provided critical information on and greater insight into the nature of biomolecules. Of significance is the application of single-molecule fluorescence resonance energy transfer (smFRET) to probe isolated events and changes at the nanometer scales. In particular, the study of helicases using smFRET has supplied much information regarding the nature and dynamics of these enzymes and provided a toolbox for further investigations. In this chapter we provide a general guide for the construction and execution of single-molecule FRET assays for the study of helicase properties and functionalities.

Original languageEnglish (US)
Title of host publicationHelicases
Subtitle of host publicationMethods and Protocols
Pages29-43
Number of pages15
DOIs
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
Volume587
ISSN (Print)1064-3745

Keywords

  • DNA
  • FRET
  • Helicase
  • binding
  • single molecule
  • translocation
  • unwinding

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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