Abstract
Six interfluorophore FRET efficiencies Eij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9922-9925 |
| Number of pages | 4 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 49 |
| Issue number | 51 |
| DOIs | |
| State | Published - Dec 17 2010 |
| Externally published | Yes |
Keywords
- DNA structures
- FRET
- conformational dynamics
- fluorescence
- single-molecule studies
ASJC Scopus subject areas
- Catalysis
- General Chemistry
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