Single-molecule four-color FRET

Jinwoo Lee, Sanghwa Lee, Kaushik Ragunathan, Chirlmin Joo, Taekjip Ha, Sungchul Hohng

Research output: Contribution to journalArticlepeer-review

Abstract

Six interfluorophore FRET efficiencies Eij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

Original languageEnglish (US)
Pages (from-to)9922-9925
Number of pages4
JournalAngewandte Chemie - International Edition
Volume49
Issue number51
DOIs
StatePublished - Dec 17 2010

Keywords

  • DNA structures
  • FRET
  • conformational dynamics
  • fluorescence
  • single-molecule studies

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry

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