Single-molecule four-color FRET

Jinwoo Lee; Sanghwa Lee; Kaushik Ragunathan; Chirlmin Joo; Taekjip Ha; Sungchul Hohng

doi:10.1002/anie.201005402

Single-molecule four-color FRET

Jinwoo Lee, Sanghwa Lee, Kaushik Ragunathan, Chirlmin Joo, Taekjip Ha, Sungchul Hohng

Research output: Contribution to journal › Article › peer-review

Abstract

Six interfluorophore FRET efficiencies E_ij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

Original language	English (US)
Pages (from-to)	9922-9925
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	51
DOIs	https://doi.org/10.1002/anie.201005402
State	Published - Dec 17 2010
Externally published	Yes

Keywords

DNA structures
FRET
conformational dynamics
fluorescence
single-molecule studies

ASJC Scopus subject areas

Catalysis
General Chemistry

Online availability

10.1002/anie.201005402

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AU - Lee, Jinwoo

AU - Lee, Sanghwa

AU - Ragunathan, Kaushik

AU - Joo, Chirlmin

AU - Ha, Taekjip

AU - Hohng, Sungchul

PY - 2010/12/17

Y1 - 2010/12/17

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AB - Six interfluorophore FRET efficiencies Eij (see scheme) can be determined in real time by a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopy. This technique was used to probe the correlated motion of the four arms of the Holliday junction, and to assess correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.

KW - DNA structures

KW - FRET

KW - conformational dynamics

KW - fluorescence

KW - single-molecule studies

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JF - Angewandte Chemie - International Edition

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Single-molecule four-color FRET

Abstract

Keywords

ASJC Scopus subject areas

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