Single molecule detection of one, two and multiplex proteins involved in DNA/RNA transaction

Yupeng Qiu, Sua Myong

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular processes involve complex arrangement of proteins engaged in a multitude of reactions, yet in a highly coordinated manner. The level of complexity, however, makes it difficult to investigate the role played by the individual protein constituent. Data taken from the conventional bulk solution methods suffer from ensemble averaging effect in which information from individual molecules is masked. The single molecule detection method overcomes this limitation by offering unique tools for monitoring the activity of individual molecules in isolation and in real-time dynamics. Included in this review are recent articles of single molecule studies representing a diverse array of experimental platforms which demonstrate the power and spectrum of single molecule detection.

Original languageEnglish (US)
Pages (from-to)125-137
Number of pages13
JournalCellular and Molecular Bioengineering
Volume4
Issue number2
DOIs
StatePublished - Jun 1 2011

Keywords

  • DNA
  • Fluorescence
  • FRET
  • Magnetic tweezers
  • Microscope
  • Polymerization
  • Protein
  • Recombination
  • Replication
  • RNA
  • Single molecule detection
  • Transcription

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

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