Single glutamate to aspartate mutation makes ferric uptake regulator (Fur) as sensitive to H2O2 as peroxide resistance regulator (PerR)

Aubérie Parent; Christelle Caux-Thang; Luca Signor; Martin Clémancey; Ramakrishnan Sethu; Geneviève Blondin; Pascale Maldivi; Victor Duarte; Jean Marc Latour

doi:10.1002/anie.201304021

Single glutamate to aspartate mutation makes ferric uptake regulator (Fur) as sensitive to H₂O₂ as peroxide resistance regulator (PerR)

Aubérie Parent, Christelle Caux-Thang, Luca Signor, Martin Clémancey, Ramakrishnan Sethu, Geneviève Blondin, Pascale Maldivi, Victor Duarte, Jean Marc Latour

Research output: Contribution to journal › Article › peer-review

Abstract

Let the Fur fly: Mutation of a single glutamate into an aspartate was shown to make the Fe sensor Fur as reactive to H₂O₂ as the peroxide sensor PerR. In vivo and in vitro peroxide sensitivities of a series of PerR and Fur Asp/Glu mutants were studied by mass spectrometry. A combination of Mössbauer spectroscopy analyses and DFT calculations gave a structural rationale for this behavior.

Original language	English (US)
Pages (from-to)	10339-10343
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	39
DOIs	https://doi.org/10.1002/anie.201304021
State	Published - Sep 23 2013
Externally published	Yes

Keywords

Mössbauer spectroscopy
density functional calculations
ferric uptake regulator
peroxide resistance regulator
peroxide stress

ASJC Scopus subject areas

Catalysis
General Chemistry

Online availability

10.1002/anie.201304021

Library availability

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Cite this

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title = "Single glutamate to aspartate mutation makes ferric uptake regulator (Fur) as sensitive to H2O2 as peroxide resistance regulator (PerR)",

abstract = "Let the Fur fly: Mutation of a single glutamate into an aspartate was shown to make the Fe sensor Fur as reactive to H2O2 as the peroxide sensor PerR. In vivo and in vitro peroxide sensitivities of a series of PerR and Fur Asp/Glu mutants were studied by mass spectrometry. A combination of M{\"o}ssbauer spectroscopy analyses and DFT calculations gave a structural rationale for this behavior.",

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AU - Parent, Aubérie

AU - Caux-Thang, Christelle

AU - Signor, Luca

AU - Clémancey, Martin

AU - Sethu, Ramakrishnan

AU - Blondin, Geneviève

AU - Maldivi, Pascale

AU - Duarte, Victor

AU - Latour, Jean Marc

PY - 2013/9/23

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KW - density functional calculations

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KW - peroxide resistance regulator

KW - peroxide stress

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