Single glutamate to aspartate mutation makes ferric uptake regulator (Fur) as sensitive to H2O2 as peroxide resistance regulator (PerR)

Aubérie Parent, Christelle Caux-Thang, Luca Signor, Martin Clémancey, Ramakrishnan Sethu, Geneviève Blondin, Pascale Maldivi, Victor Duarte, Jean Marc Latour

Research output: Contribution to journalArticle

Abstract

Let the Fur fly: Mutation of a single glutamate into an aspartate was shown to make the Fe sensor Fur as reactive to H2O2 as the peroxide sensor PerR. In vivo and in vitro peroxide sensitivities of a series of PerR and Fur Asp/Glu mutants were studied by mass spectrometry. A combination of Mössbauer spectroscopy analyses and DFT calculations gave a structural rationale for this behavior.

Original languageEnglish (US)
Pages (from-to)10339-10343
Number of pages5
JournalAngewandte Chemie - International Edition
Volume52
Issue number39
DOIs
StatePublished - Sep 23 2013
Externally publishedYes

Keywords

  • Mössbauer spectroscopy
  • density functional calculations
  • ferric uptake regulator
  • peroxide resistance regulator
  • peroxide stress

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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